Fluorescence of Naphthol AS-MX is Readily Detectable in Dioxane Mixtures

Evan D. Kindl; Kevin E. Koschnitzke; James A. Blauwkamp; Sean M. McCann; Veronica R. Moorman

doi:10.19185/matters.201905000003

Fluorescence of Naphthol AS-MX is Readily Detectable in Dioxane Mixtures

Evan D. Kindl, Kevin E. Koschnitzke, James A. Blauwkamp, Sean M. McCann, Veronica R. Moorman

Kettering University

Research output: Contribution to journal › Article › peer-review

Abstract

Numerous enzymes have been demonstrated to be active in non-aqueous solutions, yet the utility of phosphatases under such conditions has been difficult to determine. Here, we demonstrate the ability to fluorescently detect naphthol AS‑MX in high percentages 1,4-dioxane with a fluorescence differential compared with naphthol AS‑MX phosphate. While intensities and maximum fluorescence wavelengths changed depending on solvent conditions, these results demonstrate this system’s potential for testing phosphatase activity in high amounts of dioxane.

Original language	American English
Journal	Science Matters
DOIs	https://doi.org/10.19185/matters.201905000003
State	Published - May 17 2019

Disciplines

Chemistry

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10.19185/matters.201905000003License: CC BY

Fluorescence of Naphthol AS-MX is Readily Detectable in Dioxane MFinal published version, 2.1 MBLicense: CC BY

Cite this

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title = "Fluorescence of Naphthol AS-MX is Readily Detectable in Dioxane Mixtures",

abstract = " Numerous enzymes have been demonstrated to be active in non-aqueous solutions, yet the utility of phosphatases under such conditions has been difficult to determine. Here, we demonstrate the ability to fluorescently detect naphthol AS‑MX in high percentages 1,4-dioxane with a fluorescence differential compared with naphthol AS‑MX phosphate. While intensities and maximum fluorescence wavelengths changed depending on solvent conditions, these results demonstrate this system{\textquoteright}s potential for testing phosphatase activity in high amounts of dioxane.",

author = "Kindl, {Evan D.} and Koschnitzke, {Kevin E.} and Blauwkamp, {James A.} and McCann, {Sean M.} and Moorman, {Veronica R.}",

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doi = "10.19185/matters.201905000003",

language = "American English",

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T1 - Fluorescence of Naphthol AS-MX is Readily Detectable in Dioxane Mixtures

AU - Kindl, Evan D.

AU - Koschnitzke, Kevin E.

AU - Blauwkamp, James A.

AU - McCann, Sean M.

AU - Moorman, Veronica R.

PY - 2019/5/17

Y1 - 2019/5/17

N2 - Numerous enzymes have been demonstrated to be active in non-aqueous solutions, yet the utility of phosphatases under such conditions has been difficult to determine. Here, we demonstrate the ability to fluorescently detect naphthol AS‑MX in high percentages 1,4-dioxane with a fluorescence differential compared with naphthol AS‑MX phosphate. While intensities and maximum fluorescence wavelengths changed depending on solvent conditions, these results demonstrate this system’s potential for testing phosphatase activity in high amounts of dioxane.

AB - Numerous enzymes have been demonstrated to be active in non-aqueous solutions, yet the utility of phosphatases under such conditions has been difficult to determine. Here, we demonstrate the ability to fluorescently detect naphthol AS‑MX in high percentages 1,4-dioxane with a fluorescence differential compared with naphthol AS‑MX phosphate. While intensities and maximum fluorescence wavelengths changed depending on solvent conditions, these results demonstrate this system’s potential for testing phosphatase activity in high amounts of dioxane.

UR - https://digitalcommons.kettering.edu/chem_biochem_facultypubs/3

U2 - 10.19185/matters.201905000003

DO - 10.19185/matters.201905000003

M3 - Article

JO - Science Matters

JF - Science Matters

ER -

Fluorescence of Naphthol AS-MX is Readily Detectable in Dioxane Mixtures

Abstract

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