TY - JOUR
T1 - Amperometric detection after HPLC separation of selected polypeptides and proteins at an electrode modified by mixed valent ruthenium oxide crosslinked with cyanide
AU - Przyjazny, Andrzej
AU - Cox, James A.
N1 - An indicator electrode, in which a film containing Ru oxides with cyano‐crosslinks comprised the active surface, was used in an amperometric detector for ion‐exchange and reverse‐phase high‐performance liquid chromatography (HPLC). The latter used a C‐18 column and an eluent of 0.04 M citric acid, 0.1 M KH 2PO 4, 0.1% trifluoroacetic acid, and 20-80% acetonitrile.
PY - 1993/9/1
Y1 - 1993/9/1
N2 - An indicator electrode, in which a film containing Ru oxides with cyano-crosslinks comprised the active surface, was used in an amperometric detector for ion-exchange and reverse-phase high-performance liquid chromatography (HPLC). The latter used a C-18 column and an eluent of 0.04 M citric acid, 0.1 M KH2PO4, 0.1% trifluoroacetic acid, and 20–80% acetonitrile. Oxytocin, isotocin, vasopressin, tocinoic acid, myoglobin, and ribonuclease A were separated and determined with detection limits at the 1–3 pmol level. The response to ribonuclease A, as a typical example, was linear over the range of 0.050-2.5 μM, with a slope of 15 nA/μM at 0.94 V (vs. Ag/AgCl). A single preparation of the electrode was generally stable for 3 weeks, as long as the analyte concentrations were below 10 μM.
AB - An indicator electrode, in which a film containing Ru oxides with cyano-crosslinks comprised the active surface, was used in an amperometric detector for ion-exchange and reverse-phase high-performance liquid chromatography (HPLC). The latter used a C-18 column and an eluent of 0.04 M citric acid, 0.1 M KH2PO4, 0.1% trifluoroacetic acid, and 20–80% acetonitrile. Oxytocin, isotocin, vasopressin, tocinoic acid, myoglobin, and ribonuclease A were separated and determined with detection limits at the 1–3 pmol level. The response to ribonuclease A, as a typical example, was linear over the range of 0.050-2.5 μM, with a slope of 15 nA/μM at 0.94 V (vs. Ag/AgCl). A single preparation of the electrode was generally stable for 3 weeks, as long as the analyte concentrations were below 10 μM.
UR - https://onlinelibrary.wiley.com/doi/abs/10.1002/elan.1140050806
U2 - 10.1002/elan.1140050806
DO - 10.1002/elan.1140050806
M3 - Article
VL - 5
JO - Electroanalysis
JF - Electroanalysis
ER -